Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

Abstract Background A body distribution with high intramuscular fat and low abdominal is the ideal goal for broiler breeding. Preadipocytes different origins have differences in terms of metabolism gene expression. The transcriptome analysis performed this study preadipocytes (DIMFPs) adipose tissue-derived (DAFPs) aimed to explore characteristics lipid deposition chicken by dedifferentiation vitro. Results Compared DAFPs, total content DIMFPs was reduced ( P < 0.05 ). Moreover, 72 DEGs related were screened, which involved adipocyte differentiation, fatty acid transport synthesis, stabilization, lipolysis. Among DEGs, 19 enriched PPAR signaling pathway, indicating its main contribution regulation difference between DAFPs DIMFPs. these genes, representative APOA1 , ADIPOQ FABP3 FABP4 FABP7 HMGCS2 LPL RXRG genes downregulated, but ACSL1 FABP5 PCK2 PDPK1 PPARG SCD SCD5 SLC27A6 upregulated or 0.01) In addition, well-known pathways affecting (MAPK, TGF-beta calcium) cell communication enriched, may also contribute deposition. Finally, regulatory network proposed based on above information. Conclusions Our data suggested a chickens vitro molecular significantly increased direct mediation pathways. These findings provide new insights into tissue-specific optimization broilers.